This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells.
What is single cell impedance measurement?
Single-cell impedance measurement is label free and noninvasive in characterizing the electrical properties of single cells. At present, though widely used for impedance measurement, electrical impedance flow cytometry (IFC) and electrical impedance spectroscopy (EIS) are used alone for most microfluidic chips.
What is single cell impedance spectroscopy?
Impedance measurement of single cells; Impedance spectroscopy for single-cell analysis; Single-cell electrical impedance spectroscopy Single-cell impedance spectroscopy is a technique that operates by applying a frequency-dependent excitation signal on a single cell positioned in between two measurement microelectrodes.
Can impedance sensing technology be used in single-cell analysis?
Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed. 1. Introduction
What are the applications of microfluidic systems for single-cell impedance measurement?
Next, applications of two essential microfluidic systems for single-cell impedance measurement are focused: impedance flow cytometry for mobile cell detection, such as cell counting, identification, and classification, and electrical impedance spectroscopy for immobilized cell monitoring, such as cell differentiation, division, and proliferation.
What is the common theory of impedance measurement of biological cells?
Here, we discuss the common theory of impedance measurement of biological cells, and provide the typical modeling of three different sensing methods: ECIS, impedance sensing and analysis of single cells passing through a flow channel, and impedance spectroscopy of cells in suspension. 2.1. Electric model of a single cell
What is the experimental setup for electrical impedance analysis of single cells?
The most common experimental setup for electrical impedance analysis of single cells is as follows.29 AC excitation signals at different frequencies are superimposed and applied to the stimulation electrodes, to establish an electric field in the channel, which is filled with a conductive fluid.